human androgen insensitive pc3  (ATCC)

Journal:

Article Title: Carnosol, a dietary diterpene, displays growth inhibitory effects in human prostate cancer PC3 cells leading to G2-phase cell cycle arrest and targets the 5'-AMP-activated protein kinase (AMPK) pathway

doi: 10.1007/s11095-008-9552-0

Figure Lengend Snippet: Effect of carnosol on androgen insensitive prostate cancer cell line PC3. Cells were treated for 24 (◆), 48 (■) and 72 (▲) hours and cell viability was determined by MTT assay. Each concentration was repeated in three wells using a 24 well plate. Percent viable cells, where control (i.e. vehicle) were considered 100% viabile.

Article Snippet: Cell culture The androgen insensitive human prostate cancer cells PC3 obtained from the American Type Culture Collection (ATCC, Manassa, VA) were used.

Techniques: MTT Assay, Concentration Assay, Control

Journal:

Article Title: Carnosol, a dietary diterpene, displays growth inhibitory effects in human prostate cancer PC3 cells leading to G2-phase cell cycle arrest and targets the 5'-AMP-activated protein kinase (AMPK) pathway

doi: 10.1007/s11095-008-9552-0

Figure Lengend Snippet: (A) Effect of carnosol on cellular morphology in PC3 cells. Cells were grown to approximately 50% confluence and treated with vehicle (control) and carnosol (20 – 60 µM) for 48 hours using phase contrast microscopy. (B) Effect of carnosol on cell cycle regulation leading to G2 cell cycle arrest in PC3 cells. Cells were treated with carnosol for 48 hours, collected, and labeled using the APODIRECT kit per vendor’s protocol as detailed in materials and methods. (C) Carnosol displays a dose dependent effect on cell cycle regulatory proteins in PC3 cells. As detailed in materials and methods, whole cell lysates were prepared and the protein was subjected to SDS-PAGE followed by immunoblot anlaysis and chemiluminescence detection. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-actin.

Article Snippet: Cell culture The androgen insensitive human prostate cancer cells PC3 obtained from the American Type Culture Collection (ATCC, Manassa, VA) were used.

Techniques: Control, Microscopy, Labeling, SDS Page, Western Blot, Stripping Membranes

Journal:

Article Title: Carnosol, a dietary diterpene, displays growth inhibitory effects in human prostate cancer PC3 cells leading to G2-phase cell cycle arrest and targets the 5'-AMP-activated protein kinase (AMPK) pathway

doi: 10.1007/s11095-008-9552-0

Figure Lengend Snippet: PC3 cells were treated with carnosol (40 µM) and a protein array was analyzed for proteins that are up regulated at least 200% compared to control.

Article Snippet: Cell culture The androgen insensitive human prostate cancer cells PC3 obtained from the American Type Culture Collection (ATCC, Manassa, VA) were used.

Techniques: Protein Array, Control

Journal:

Article Title: Carnosol, a dietary diterpene, displays growth inhibitory effects in human prostate cancer PC3 cells leading to G2-phase cell cycle arrest and targets the 5'-AMP-activated protein kinase (AMPK) pathway

doi: 10.1007/s11095-008-9552-0

Figure Lengend Snippet: (A) Effect of carnosol on mitochondrial dependent apoptotic proteins in PC3 cells. As detailed in materials and methods, whole cell lysates were prepared and the protein was subjected to SDS-PAGE followed by immunoblot anlaysis and chemiluminescence detection. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-actin. (B) PC3 cells treated with carnosol were labeled as described in materials and methods and apoptosis was quantified by flow cytometry.

Article Snippet: Cell culture The androgen insensitive human prostate cancer cells PC3 obtained from the American Type Culture Collection (ATCC, Manassa, VA) were used.

Techniques: SDS Page, Western Blot, Stripping Membranes, Labeling, Flow Cytometry

Journal:

Article Title: Carnosol, a dietary diterpene, displays growth inhibitory effects in human prostate cancer PC3 cells leading to G2-phase cell cycle arrest and targets the 5'-AMP-activated protein kinase (AMPK) pathway

doi: 10.1007/s11095-008-9552-0

Figure Lengend Snippet: PC3 cells were treated with carnosol (40 µM) and a protein array was analyzed for proteins that are down regulated at least 50% compared to control.

Article Snippet: Cell culture The androgen insensitive human prostate cancer cells PC3 obtained from the American Type Culture Collection (ATCC, Manassa, VA) were used.

Techniques: Protein Array, Control

Journal:

Article Title: Carnosol, a dietary diterpene, displays growth inhibitory effects in human prostate cancer PC3 cells leading to G2-phase cell cycle arrest and targets the 5'-AMP-activated protein kinase (AMPK) pathway

doi: 10.1007/s11095-008-9552-0

Figure Lengend Snippet: Proposed mechanism of carnosol in human prostate cancer PC3 cell line.

Article Snippet: Cell culture The androgen insensitive human prostate cancer cells PC3 obtained from the American Type Culture Collection (ATCC, Manassa, VA) were used.

Techniques: